If egg fertilisation (conception) is successful, then around 12 days later, the pregnancy hormone human Chorionic Gonadotropin (hCG) level will begin to increase. The myLotus Monitor will calculate your hCG testing day based on your testing and the detection of your LH surge. All you need to do is to look at your Monitor to see if an hCG test is requested. It is usually requested 12 days after a ‘+’ LH measurement or LH surge. If no pregnancy is detected the Monitor will ask you for another pregnancy test 2 days later and again 2 days after that.You also have the personal choice to do an hCG test at any time you wish.